Identification of the different parts of an embryo of a dicot seed (Pea, gram or red kidney bean)
Aim
To identify the different parts of an embryo of a dicot seed (pea, gram or red kidney
bean).
Materials Required
Seeds of bengal gram/red kidney bean, forceps, cloth, magnifying glass, petridish, water.
Theory
Procedure
- Soak a few bean seeds and keep them overnight.
- Drain the excess water.
- Cover the seeds with a moist cloth and keep them aside for a day.
- Peel off the seed coat carefully.
- Using your thumbs or the forceps, open the bean seed so that it falls into two halves.
- With the help of the magnifying glass, locate the parts of the bean embryo carefully.
- Draw a sketch of the inside of the dicot seed that you examined. Label all its parts.
Observations
- Been seed is kidney-shaped with a concave and convex side.
- The concave side which is slightly darker has a scar called hilum.
- Adjacent to hilum a small pore known as micropyle is present.
- The seed is covered by a seed coat.
- The embryo consists of two large, white and kidney-shaped cotyledons.
- The cotyledons are attached laterally to the curved embryonal axis.
- Rod shaped and slightly bulgy lower end of embryonal axis which lies towards micropylar end is called radicle.
- Plumule is the upper end of embryonal axis.
- The part of embryo axis between radicle and attachment of cotyledon leaves is called hypocotyl.
- The part of embryo axis between plumule and attachment of cotyledon leaves is called epicotyl.
Result
- The embryo of dicot seeds consists of three main parts – plumule, radicle and two cotyledons.
Precautions
- The cloth which is used to cover seeds should be moist and should not get dry.
- Seeds should be open carefully so that the embryo does not get damaged.
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- Identification of the different parts of an embryo of a dicot seed (Pea, gram or red kidney bean)
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