Studying (a) binary fission in Amoeba, and (b) budding in yeast with the help of prepared slides


Aim

To study (a) binary fission in Amoeba, and (b) budding in yeast with the help of prepared slides.

Materials Required

Compound microscope, permanent slides of binary fission in Amoeba and budding in yeast.

Theory

Binary Fission – This is a type of asexual reproduction which takes place in Amoeba. In this type of reproduction, parent cell divides into two small, nearly equal sized identical daughter cells. The two daughter cells then grow into adult organism. In binary fission parental identity is lost.

Budding – This is also a type of asexual reproduction which is common in yeast. In this type of reproduction a small bud-like outgrowth develops at tḥe parent cell. This bud enlarges in size. The nucleus also enlarges in size and divides to form two nuclei. Out of these two nuclei, one goes into developing bud. When the bud attains almost the same size as that of parent cell, a separating wall is laid down between the bud and parent cell. This bud may detach from the parent cell or may produce another bud over it.

Procedure

  • Select the slide of binary fission in Amoeba and budding in Yeast.
  • Set-up a compound microscope. While looking through the eye piece, adjust the mirror and diaphragm so that microscope is illuminated.
  • Now, place the slide on stage and focus at low power. Observe it.
  • Without removing the slide, now change the focus to high power. Observe it.

Observations

(a) Binary Fission in Amoeba

  • Thiis is a type of asexual reproduction in which two daughter cells (or two individuals) are formed from a single parent.
  • Parent cell becomes elongated.
  • Nucleus divides first and then the cytoplasm divides.
  • At the point of fission, construction appears and deepens to divide the cell into two daughter cells.

(b) Budding in Yeast

  • In this type of asexual reproduction, a small protuberance or outgrowth arises from the parent body called bud.
  • Nucleus divides to form two daughter nuclei, of which one passes into the bud.
  • The bud now detaches from the parent body and grows independently as a new individual or may remain attached to parent body, forming chain of cells.
  • Parental identity is not lost.

Result

The slides show reproduction by binary fission in Amoeba and budding in yeast.

Precautions

  • Always focus the slide, first at low power and then at high power.
  • While focussing at high power, do not use coarse adjustment of microscope. Only use fine adjustment.

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